Quite often, in order to determine the concentration of a molecule in a mixture or solution, or to determine the purity of material, itís simply enough to run an assay with a UV-VIS or Luminescence/Fluorescence, or Diode Array Spectrophotometer. The principle of working of these instruments is simply to emit an electromagnetic radiation to the sample and to detect the wavelength and the intensity of absorbance and/or emittance, by the sample. The wavelength gives valuable information about the chemical structure, neighboring moieties to a certain substrates and the intensity, is related to the number of molecules, means quantity or concentration. Therefore, these methods are excellent to both determine the concentration and identify the molecular structure or the structural changes. Spectrophotometers are also useful to study the changes in the vibration and conformation energy levels after and before an interaction with a substrate, or another molecule.


In our spectrophotometer facility, we have:


         A Perkin Elmer LS4 Luminescence/Fluorescence Spectrophotometer

         A Perkin-Elmer 1420 Infared (IR) Spectrophotometer

         An Eppendorf BT 9550 UV-VIS Spectrophotometer

         A Waters F.S. 970 LC Fluorescence Spectrophotometer

         A HIAC ROYCO Nicomp 270 Photon Correlation Spectrophotometer

         A Malvern 4700c Photon Correlation Spectrophotometer

         A PE SCIEX API Quadropole Mass Spectrometer LC/MS System


We are also planning to purchase a Diode Array, Atomic Absorption and a bigger UV-VIS Spectrophotometer.


























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